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CAS#
規格
單價
說明書
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100ug
產品說明
Catalog No. PGM3005 Size 100ug Source Mouse Immunogen Synthetic Peptide Purification The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. Specificity The antibody detects C-terminal, internal, and N-terminal V5-tag fusion proteins. Formulation PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. Concen… 【產品詳情】
產品說明
取200mg幼嫩葉片, 裝入2ml離心管,加液氮研磨(加鋼珠或玻璃珠在振蕩器中處理5分鐘);向上述研磨過的樣品中,加植物組織抽提液100ul(如果加500mg葉片,則需要用250ul抽提液), 研磨2分鐘(具體做法是:加鋼珠或玻璃珠在振蕩器中處理5分鐘);12000rpm離10分鐘,4℃;用移液器吸取上清(一般是80-120ul),轉移至新的1.5ml離心管,做好標記;加入1/3體積的5x SDS loading buffer (for PAGE),混勻,100℃ 處理… 【產品詳情】